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Updated: Sep 5, 2025

Transmission Electron Microscopy as the Visualization Technique for Analysis of Circadian Synaptic Plasticity in the Mouse Barrel Cortex
Published on: August 19, 2025
Electron Tomographic Methods for Studying Organelles of the Murine Chemical Synapse
Tamara Basta1, Garry P Morgan2, Eileen T O'Toole2
1Department of Molecular, Cellular & Developmental Biology, University of Colorado, Boulder, CO, USA.
Abstract:
Electron tomography of the chemical synapse provides important architectural information regarding the organization of synaptic organelles including synaptic vesicles, Nissl bodies, and early endosomes. Here, we describe methods for the preparation of select murine brain regions for high-pressure freezing, freeze substitution, and EM tomographic analysis of synaptic structures. The method uses fresh brain slices prepared using a vibratome and biopsy punches to collect specific brain regions of interest suitable for subsequent preservation and EM tomographic imaging.
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