Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA as a Genetic Template02:05

DNA as a Genetic Template

22.6K
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
22.6K
The Replisome03:01

The Replisome

34.6K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
34.6K
Nucleic Acid Structure01:25

Nucleic Acid Structure

6.6K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
6.6K
The DNA Helix01:07

The DNA Helix

23.0K
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
23.0K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.4K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.4K
Phosphodiester Linkages01:01

Phosphodiester Linkages

102.4K
Overview
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
102.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Disruption of microtubules with low intensity ultrasound rescues hair follicle damage by paclitaxel in mouse models.

Nature communications·2026
Same author

Innate Immunity of Framework Nucleic Acids.

Accounts of chemical research·2026
Same author

A multiple-encrypted DNA device for secure communication.

Science advances·2026
Same author

Characteristics, Clinical Manifestations, and Severe Disease Risk of Bordetella pertussis Infection in Children: A tNGS-based Retrospective Study.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026
Same author

Programmable Hierarchical Assembly of Atomically Precise Metal Nanoclusters Using Supra-Amphiphilic Nucleic Acids.

JACS Au·2026
Same author

Programming Dimensional Transitions in DNA Brick Crystals via Interfacial Connectivity.

Angewandte Chemie (International ed. in English)·2026

Related Experiment Video

Updated: Sep 4, 2025

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

11.8K

Programming the self-assembly of amphiphilic DNA frameworks for sequential boolean logic functions.

Chengpin Liang1, Jielin Chen1, Mingqiang Li1

  • 1School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China. fanchunhai@sjtu.edu.cn.

Chemical Communications (Cambridge, England)
|July 15, 2022
PubMed
Summary
This summary is machine-generated.

Researchers used orthogonal noncovalent interactions to program self-assembling amphiphilic DNA frameworks. This enabled the creation of DNA-based logic gates, including primary (NOT, AND, OR, INH) and secondary (NOT-OR) gates.

More Related Videos

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.3K
Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

891

Related Experiment Videos

Last Updated: Sep 4, 2025

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
10:23

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Published on: May 8, 2015

11.8K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.3K
Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures
08:02

Author Spotlight: Developing Synthetic Cells from Programmable Amphiphilic DNA Nanostructures

Published on: May 31, 2024

891

Area of Science:

  • Biomolecular Engineering
  • Nanotechnology
  • Synthetic Biology

Background:

  • Amphiphilic DNA frameworks (am-FNAs) offer potential for nanoscale construction.
  • Programming self-assembly is key to creating functional DNA-based nanostructures.
  • Orthogonal noncovalent interactions provide precise control over molecular assembly.

Purpose of the Study:

  • To investigate the use of orthogonal noncovalent interactions for programming am-FNA self-assembly.
  • To construct DNA-based logic gates using self-assembled am-FNAs.
  • To demonstrate the creation of both primary and secondary logic gates.

Main Methods:

  • Utilizing orthogonal noncovalent interactions to direct DNA self-assembly.
  • Fine-tuning reaction parameters: ionic strength, amphiphilic DNA length, and mechanical agitation.
  • Fabricating primary logic gates (NOT, AND, OR, INH) and a secondary logic gate (NOT-OR).

Main Results:

  • Successful programming of am-FNA self-assembly through controlled orthogonal noncovalent interactions.
  • Construction of functional DNA-based primary logic gates (NOT, AND, OR, INH).
  • Demonstrated fabrication of a secondary logic gate (NOT-OR) using the same framework.

Conclusions:

  • Orthogonal noncovalent interactions are effective for programming amphiphilic DNA framework self-assembly.
  • This approach enables the creation of complex DNA-based logic circuits.
  • The developed am-FNA logic gates hold promise for applications in molecular computing and sensing.