Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

fastdemux: Robust SNP-based demultiplexing of single-cell population genomics data.

bioRxiv : the preprint server for biology·2026
Same author

Host genotypes interact with microbial communities to modulate gene expression in the human intestine.

medRxiv : the preprint server for health sciences·2026
Same author

TMEM120A maintains adipose tissue lipid homeostasis through ER CoA channeling.

Nature communications·2025
Same author

Spatial Transcriptomics of Adipose Tissue: Technologies, Applications, and Challenges.

Journal of obesity & metabolic syndrome·2025
Same author

Living in poverty is associated with gene expression changes in immune cells.

Genetics·2025
Same author

Homeostatic Macrophages Prevent Preterm Birth and Improve Neonatal Outcomes by Mitigating In Utero Sterile Inflammation in Mice.

Journal of immunology (Baltimore, Md. : 1950)·2024

Related Experiment Video

Updated: Sep 4, 2025

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
04:46

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

1.6K

Deconstructing cold-induced brown adipocyte neogenesis in mice.

Rayanne B Burl1, Elizabeth Ann Rondini1,2, Hongguang Wei1,2

  • 1Center for Molecular Medicine and Genetics, Wayne State University, Detroit, United States.

Elife
|July 18, 2022
PubMed
Summary

Cold exposure induces brown adipocyte (BA) neogenesis, but not through direct beta-adrenergic signaling on progenitors. Instead, mature BAs signal indirectly, involving immune cell interactions, to drive new BA formation.

Keywords:
adipose stromal cellsbrown adipose tissuecell biologyimmune cellsmouseplasticitysingle-cell RNA-seq

More Related Videos

Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging
08:31

Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging

Published on: July 1, 2021

3.2K
Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice
09:00

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice

Published on: January 25, 2021

11.9K

Related Experiment Videos

Last Updated: Sep 4, 2025

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
04:46

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

1.6K
Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging
08:31

Visualization and Quantification of Brown and Beige Adipose Tissues in Mice using [18F]FDG Micro-PET/MR Imaging

Published on: July 1, 2021

3.2K
Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice
09:00

Isolation and Differentiation of Primary White and Brown Preadipocytes from Newborn Mice

Published on: January 25, 2021

11.9K

Area of Science:

  • Cell biology
  • Adipose tissue biology
  • Immunology

Background:

  • Cold exposure stimulates brown adipose tissue (BAT) neogenesis.
  • This process involves adipose tissue stromal cells (ASCs) and immune cell recruitment.
  • The precise mechanisms and cell-cell interactions remain unclear.

Purpose of the Study:

  • To elucidate the cellular and molecular mechanisms of cold-induced brown adipocyte neogenesis.
  • To identify the precursors of new brown adipocytes and the signaling pathways involved.
  • To investigate the role of immune cells in this adaptive process.

Main Methods:

  • Single-cell RNA-sequencing (scRNA-seq) in mice.
  • Genetic manipulation (receptor knockouts).
  • Pharmacological activation of adrenergic receptors.
  • High-resolution immunofluorescence and single-molecule fluorescence in situ hybridization.

Main Results:

  • Identified three ASC subpopulations, with ASC1 as direct brown adipocyte precursors.
  • Beta-1 adrenergic receptor knockout in ASCs did not inhibit neogenesis.
  • Beta-3 adrenergic receptor activation in mature brown adipocytes was sufficient to trigger neogenesis indirectly.
  • Cold exposure led to delayed recruitment of macrophages and dendritic cells, correlating with neogenesis.
  • Observed dynamic, micrometer-scale interactions between ASC1 and immune cells.

Conclusions:

  • Brown adipocyte neogenesis is not a direct response to beta-adrenergic signaling on progenitors.
  • Mature brown adipocytes indirectly regulate neogenesis via signals that involve immune cell recruitment.
  • Neogenesis is a complex adaptive response to metabolic demand, mediated by intricate cell-cell interactions.