Single DNA Origami Detection by Nanoimpact Electrochemistry

DNA has emerged as the material of choice for producing supramolecular building blocks of arbitrary geometry from the 'bottom up'. Characterisation of these structures via electron or atomic force microscopy usually requires their surface immobilisation. In this work, we developed a nanoimpact electrochemistry platform to detect DNA self-assembled origami structures in solution, using the intercalator methylene blue as a redox probe. Here, we report the electrochemical detection of single DNA origami collisions at Pt microelectrodes. Our work paves the way towards the characterisation of DNA nanostructures in solution via nanoimpact electrochemistry.