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Steps for the Autologous Ex vivo Perfused Porcine Liver-kidney Experiment

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Liposome Formulation and In Vitro Testing in Non-Physiological Conditions Addressed to Ex Vivo Kidney Perfusion.

Silvia Pisani¹, Enrica Chiesa², Ida Genta²

¹Otorhinolaryngology Unit, Department of Surgical Sciences, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy.

International Journal of Molecular Sciences

|July 27, 2022

Summary

Microfluidics effectively create liposomes for kidney preservation during machine perfusion. These liposomes deliver proteins, enhancing organ viability and reducing injury in transplantable kidneys.

Keywords:

drug delivery kidney liposomes protein delivery transplant

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Last Updated: Sep 3, 2025

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Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation

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The Mouse Isolated Perfused Kidney Technique

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Area of Science:

Biomedical Engineering
Organ Transplantation
Drug Delivery Systems

Background:

Marginal donor kidneys face higher ischemic/reperfusion injury risk.
Improved preservation strategies are crucial for transplantable organs.
Liposomes offer potential as targeted drug delivery systems.

Purpose of the Study:

Formulate liposomes for hypothermic machine perfusion in kidney preservation.
Evaluate liposome preparation techniques for drug delivery.
Assess protein release and cellular uptake for enhanced organ protection.

Main Methods:

Liposome formulation via thin film hydration and microfluidics.
Characterization using Dynamic Light Scattering (DLS) and Transmission Electron Microscopy (TEM).
In vitro and ex vivo protein release studies in perfusion fluid and cell cultures.

Main Results:

Microfluidics yielded reproducible spherical liposomes (<200 nm).
Protein encapsulation efficiency correlated with molecular weight and isoelectric point.
Lower temperatures and specific perfusion fluids modulated protein release.

Conclusions:

Microfluidic liposomes are suitable for hypothermic kidney perfusion.
Liposome formulation parameters influence drug delivery efficacy.
Liposomes demonstrate uptake by renal tubular cells, indicating therapeutic potential.