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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
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Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...

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Microbe-Based Sensor for Long-Term Detection of Urine Glucose.

Dunzhu Li1, Yunhong Shi1, Yifan Sun1

  • 1Department of Civil, Structural and Environmental Engineering, Trinity College Dublin, D02 PN40 Dublin, Ireland.

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|July 27, 2022
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Summary

A new, affordable urine glucose sensor using microbial fuel cells (MFCs) offers continuous diabetes monitoring. This reusable sensor demonstrates high accuracy and stability, improving diabetes management.

Keywords:
diabetes pre-screeningglucose sensormicrobial fuel cellselectivityurine glucose

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Environmental Science

Background:

  • Diabetes mellitus requires continuous glucose monitoring for effective management.
  • Current urine glucose test strips lack reusability and long-term stability.
  • Microbial fuel cells (MFCs) offer potential for developing selective biosensors.

Purpose of the Study:

  • To develop and assess a novel microbial fuel cell-based cylinder sensor (CS) for continuous urine glucose monitoring.
  • To evaluate the sensor's performance, including response time, detection range, accuracy, and stability.
  • To determine the sensor's selectivity and compare its results with a commercial glucose meter.

Main Methods:

  • Fabrication of a novel cylinder sensor (CS) utilizing MFC technology.
  • Testing sensor performance with synthetic and actual urine samples (diabetes-negative and positive).
  • Optimizing sensor selectivity by adjusting culture organic matter concentration.
  • Comparative analysis with a commercial glucose meter.

Main Results:

  • The CS exhibited a rapid response time (100 s) and a wide detection range (0.3–5 mM).
  • Sensor stability was demonstrated, lasting up to 5 months with excellent accuracy.
  • Selectivity was confirmed and enhanced by adjusting culture conditions; results correlated well with a commercial meter (93.6%–127.9% recovery).

Conclusions:

  • The developed MFC-based CS is a low-cost, reusable, and highly sensitive sensor for urine glucose.
  • It offers significant advantages in stability and sensitivity over traditional urine test strips.
  • This novel sensor provides a reliable approach for continuous urine glucose monitoring, aiding diabetes assessment and control.