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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Sep 3, 2025

Retzius-Sparing Robot-Assisted Radical Prostatectomy

Retzius-Sparing Robot-Assisted Radical Prostatectomy

Published on: May 19, 2022

Fluorescence-guided radical prostatectomy.

Osamah Hasan¹, Alexandra Reed², Mohammed Shahait^3,4

¹Department of Surgery, Division of Urology, Cook County Health, Chicago, IL, USA. osamah.hasan@midwestern.edu.

International Urology and Nephrology

|July 29, 2022

Summary

This summary is machine-generated.

Indocyanine green (ICG) fluorescence enhances visualization during robotic prostatectomy, improving surgical precision. This review evaluates evidence for ICG-guided surgery and its future potential in urologic oncology.

Keywords:

Fluorescence Indocyanine green Prostate Prostatectomy Prostatic neoplasms

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Last Updated: Sep 3, 2025

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Area of Science:

Urologic Oncology
Surgical Technology

Background:

Robotic-assisted laparoscopic radical prostatectomy is a common procedure with critical steps impacting patient outcomes.
Effective visualization of anatomical structures and vasculature is crucial for surgical success.

Purpose of the Study:

To review the existing evidence on the utility of Indocyanine green (ICG) fluorescence in robotic prostatectomy.
To explore future research directions for ICG fluorescence technology in this surgical context.

Main Methods:

Systematic review of studies investigating ICG fluorescence during robotic prostatectomy.
Evaluation of the role of ICG in enhancing visualization of critical surgical steps.

Main Results:

ICG fluorescence provides enhanced near-infrared visualization of anatomical landmarks and blood vessels.
Evidence suggests potential benefits for ICG in improving the performance of key surgical maneuvers.

Conclusions:

Fluorescence-guided robotic prostatectomy using ICG shows promise for improving surgical quality.
Further research is warranted to fully elucidate the benefits and optimize the application of ICG in prostatectomy.