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Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Updated: Sep 2, 2025

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

A High-throughput Cell Microarray Platform for Correlative Analysis of Cell Differentiation and Traction Forces

Published on: March 1, 2017

Topology optimization based deterministic lateral displacement array design for cell separation.

Hao Tang¹, Jiaqi Niu¹, Xinni Pan²

¹Shanghai Jiao Tong University, Shanghai, China.

Journal of Chromatography. A

|August 8, 2022

Summary

This summary is machine-generated.

This study introduces a novel microfluidic chip using topology optimization for efficient circulating tumor cell (CTC) isolation. The design leverages an altered zigzag mode for precise cell manipulation, improving cancer theranostics.

Keywords:

Altered zigzag mode Circulating tumor cell Deterministic lateral displacement Pillar shape Topology optimization

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Last Updated: Sep 2, 2025

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Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

Area of Science:

Biomedical Engineering
Microfluidics
Cell Separation Technology

Background:

Circulating tumor cells (CTCs) are crucial biomarkers for cancer theranostics.
Efficient isolation of CTCs from blood is a significant clinical challenge.
Deterministic Lateral Displacement (DLD) microfluidics offers a passive method for cell separation based on size.

Purpose of the Study:

To develop a novel DLD microfluidic chip for enhanced CTC isolation.
To utilize the altered zigzag mode in DLD for targeted cell manipulation.
To employ topology optimization (TO) for designing an efficient DLD array and channel.

Main Methods:

Design of a DLD chip with topology-optimized pillar shapes and a wide channel.
Investigation and utilization of the altered zigzag mode for particle manipulation.
Experimental validation using bead and cell separation tests, including critical diameter determination.

Main Results:

The topology optimization method significantly enhanced lateral displacement for cells of varying sizes.
The novel DLD chip demonstrated improved separation efficiency.
The altered zigzag mode was successfully employed for particle manipulation, a first in DLD applications.

Conclusions:

The TO-based DLD chip design offers a promising approach for efficient CTC isolation.
This method provides a versatile and expandable platform for cell manipulation.
The developed technology has the potential to advance cancer diagnostics and theranostics.