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Updated: Sep 1, 2025

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

Published on: September 22, 2020

Monkeypox: another test for PCR.

Jim F Huggett^1,2, David French¹, Denise M O'Sullivan¹

¹National Measurement Laboratory, LGC, Teddington, United Kingdom.

Euro Surveillance : Bulletin Europeen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin

|August 12, 2022

Summary

This summary is machine-generated.

Standardizing PCR testing for monkeypox virus is crucial for accurate public health response. Early attention to PCR assay accuracy and harmonization will ensure reliable detection as global testing needs grow.

Keywords:

Monkeypox PCR molecular diagnosis standardisation test accuracy

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Last Updated: Sep 1, 2025

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Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

Absolute Quantification of Plasma MicroRNA Levels in Cynomolgus Monkeys, Using Quantitative Real-time Reverse Transcription PCR

Published on: February 12, 2018

Area of Science:

Virology
Public Health
Molecular Diagnostics

Background:

Monkeypox declared a global health emergency by WHO on July 23, 2022.
Over 16,000 cases reported globally between January and July 2022.
Public health efforts focus on case identification and contact tracing.

Purpose of the Study:

To highlight the importance of standardizing PCR assays for monkeypox.
To advocate for early attention to PCR accuracy and harmonization.
To ensure reliable diagnostic testing for public health interventions.

Main Methods:

Polymerase Chain Reaction (PCR) is identified as the primary diagnostic method.
Discussion on the current limited standardization of PCR assays.
Emphasis on the need for standardized materials, documentation, and methodologies.

Main Results:

PCR is essential for rapid feedback on public health interventions.
Limited information currently exists on inter-laboratory standardization of monkeypox PCR.
Detection, not quantification, is the primary clinical requirement for monkeypox DNA.

Conclusions:

Complacency regarding PCR performance is unwarranted.
Ensuring PCR accuracy from the outset is prudent as testing scales up.
Lessons from COVID-19 emphasize the need for robust standards in diagnostic testing.