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Updated: Sep 1, 2025

Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues
Published on: May 26, 2018
An interactive metabolic map of bio-based chemicals
Woo Dae Jang1, Gi Bae Kim2, Sang Yup Lee1
1Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 four), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Systems Metabolic Engineering and Systems Healthcare Cross-Generation Collaborative Laboratory, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; BioProcess Engineering Research Center and BioInformatics Research Center, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
This study introduces an interactive map for bio-based chemical production, visualizing metabolic pathways, enzymes, and compounds. It aids in understanding and optimizing the synthesis of chemicals using biological and chemical methods.
Area of Science:
- Biotechnology and metabolic engineering
- Chemical synthesis and pathway analysis
Background:
- Metabolic engineering for bio-based chemical production necessitates a deep understanding of complex metabolic reactions.
- Key components include enzymes, cofactors, reactants, and products, which are crucial for pathway elucidation.
Purpose of the Study:
- To present an interactive map visualizing compounds, enzymes, and reaction pathways for bio-based chemical production.
- To integrate strategies for chemical production using biological, chemical, and combined approaches.
Main Methods:
- Development of an interactive visualization tool.
- Integration of data on metabolic compounds, enzymes, and reaction pathways.
- Inclusion of diverse production strategies (biological, chemical, combined).
Main Results:
- A comprehensive, interactive map illustrating metabolic networks and chemical production routes.
- Visualization of the interplay between compounds, enzymes, and pathways.
- Consolidated information on various production strategies.
Conclusions:
- The interactive map serves as a valuable resource for metabolic engineering and bio-based chemical production.
- Enhanced understanding and optimization of chemical synthesis through integrated visualization.
- Facilitates the exploration of novel production strategies by combining different methods.
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