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Published on: June 11, 2015

Staphylococcal adherence to polyvinyl chloride and heparin-bonded polyurethane catheters is species dependent and

P B Russell, J Kline, M C Yoder

    Journal of Clinical Microbiology
    |June 1, 1987
    PubMed

    Insights

    Catheter material affects bacterial adherence, influencing sepsis risk in premature infants receiving intravenous nutrition. Fibronectin coating alters bacterial binding, highlighting the role of catheter composition in nosocomial infections.

    Area of Science:

    • Medical Microbiology
    • Biomaterials Science
    • Neonatology

    Background:

    • Intravenous hyperalimentation improves survival in premature infants.
    • Long-term intravenous catheter use increases the risk of catheter-related sepsis.
    • Plasma fibronectin may promote bacterial adherence to indwelling catheters.

    Purpose of the Study:

    • To investigate the adherence of staphylococci to different catheter materials.
    • To determine the effect of fibronectin coating on bacterial adherence.
    • To assess fibronectin binding to catheter surfaces.

    Main Methods:

    • Labeled coagulase-positive (CPS) and coagulase-negative (CNS) staphylococci adherence assays.
    • Quantification of fibronectin binding to polyvinyl chloride (PVC) and heparin-bonded polyurethane (HBP) catheter segments.
    • Comparison of bacterial adherence and fibronectin binding on untreated and fibronectin-coated catheters.

    Main Results:

    • PVC catheters showed higher CNS adherence than CPS.
    • HBP catheters demonstrated higher CPS adherence than CNS.
    • Fibronectin coating increased CPS adherence to PVC and CNS adherence to HBP.
    • PVC catheters exhibited significantly greater fibronectin binding than HBP catheters.

    Conclusions:

    • Catheter material influences the adherence spectrum of staphylococcal species.
    • Fibronectin coating modifies bacterial adherence patterns on catheters.
    • Catheter composition plays a role in determining infant susceptibility to nosocomial pathogens via bacterial adherence and protein interactions.

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