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High-throughput microarray reveals the epitranscriptome-wide landscape of m6A-modified circRNA in oral squamous cell
Wei Zhao1, Jingwen Liu1, Jie Wu1
1The School and Hospital of Stomatology, Tianjin Medical University, 300070, Tianjin, China.
Background:
Emerging transcriptome-wide high-throughput screenings reveal the landscape and functions of RNAs, such as circular RNAs (circRNAs), in human cancer. In addition, the post-transcriptional RNA internal modifications, especially N6-methyladenosine (m6A), greatly enrich the variety of RNAs metabolism. However, the m6A modification on circRNAs has yet to be addressed.
Results:
Here, we report an epitranscriptome-wide mapping of m6A-modified circRNAs (m6A-circRNA) in oral squamous cell carcinoma (OSCC). Utilizing the data of m6A methylated RNA immunoprecipitation sequencing (MeRIP-seq) and m6A-circRNAs microarray, we found that m6A-circRNAs exhibited particular modification styles in OSCC, which was independent of m6A-mRNA. Besides, m6A modification on circRNAs frequently occurred on the long exons in the front part of the coding sequence (CDS), which was distinct from m6A-mRNA that in 3'-UTR or stop codon.
Conclusion:
In conclusion, our work preliminarily demonstrates the traits of m6A-circRNAs, which may bring enlighten for the roles of m6A-circRNAs in OSCC.
Insights
This study maps N6-methyladenosine (m6A) modifications on circular RNAs (circRNAs) in oral squamous cell carcinoma (OSCC). These m6A-circRNAs show unique modification patterns distinct from m6A-messenger RNAs (mRNAs).
Area of Science:
- Molecular Biology
- Cancer Research
- Epitranscriptomics
Background:
- High-throughput screenings reveal RNA functions, including circular RNAs (circRNAs), in human cancer.
- N6-methyladenosine (m6A) modifications diversify RNA metabolism.
- The m6A modification on circRNAs remains largely unaddressed.
Purpose of the Study:
- To perform epitranscriptome-wide mapping of m6A-modified circRNAs (m6A-circRNAs).
- To investigate the characteristics of m6A modification on circRNAs in oral squamous cell carcinoma (OSCC).
Main Methods:
- Utilized m6A methylated RNA immunoprecipitation sequencing (MeRIP-seq).
- Employed m6A-circRNAs microarray analysis.
- Analyzed m6A modification patterns in OSCC.
Main Results:
- m6A-circRNAs exhibit distinct modification styles in OSCC, independent of m6A-mRNAs.
- m6A modification on circRNAs preferentially occurs on long exons within the coding sequence (CDS).
- This pattern differs from m6A-mRNAs, which are typically modified in the 3'-untranslated region (3'-UTR) or near the stop codon.
Conclusions:
- This work provides preliminary insights into the traits of m6A-circRNAs.
- Findings may illuminate the roles of m6A-circRNAs in OSCC pathogenesis.
