[Advances of CRISPR/Cas9 activation system]

Area of Science:

• Biotechnology
• Molecular Biology
• Genetics

Background:

• CRISPR/Cas systems are highly specific, simple, and flexible gene editing tools.
• Transcriptional activators combined with deactivated Cas protein allow targeted gene expression regulation.
• Overexpressing multiple genes is crucial for function verification but limited by vector capacity.

Purpose of the Study:

• To review the CRISPR/Cas9 activation system's composition and activation strategies.
• To summarize solutions for addressing excessive activation.
• To highlight potential applications in cotton genetic improvement and herbicide resistance.

Main Methods:

• Review of CRISPR/Cas9 activation system components.
• Analysis of various gene activation strategies.
• Discussion of methods to control and mitigate overactivation.

Main Results:

• CRISPR/Cas9 activation systems can regulate multiple genes using different guide RNAs.
• This system facilitates gene function verification at the regulatory level.
• The review provides insights into managing activation levels.

Conclusions:

• CRISPR/Cas9 activation systems offer a powerful tool for complex gene regulation.
• This technology has significant potential for agricultural biotechnology, particularly in cotton.
• Further research can optimize its use for genetic improvement and herbicide resistance studies.