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Updated: Aug 30, 2025
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Determination of Biofilm Initiation on Virus-infected Cells by Bacteria and Fungi
Published on: July 6, 2016
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Interactions Between Infectious Foodborne Viruses and Bacterial Biofilms Formed on Different Food Contact Surfaces
Marie-Josée Gagné1, Tony Savard1, Julie Brassard2
1Agriculture and Agri-Food Canada, Saint-Hyacinthe Research and Development Centre, 3600 Casavant Boulevard West, Saint-Hyacinthe, QC, J2S 8E3, Canada.
Food and Environmental Virology
|August 27, 2022
Summary
Bacterial biofilms significantly increase enteric virus attachment to food contact surfaces, particularly rotavirus and hepatitis A virus. This association impacts food safety and hygiene, though influencing factors remain unclear.
Area of Science:
- Food Microbiology
- Virology
- Surface Science
Background:
- Bacterial biofilms are implicated in food industry contamination and hygiene failures.
- Limited knowledge exists on enteric virus behavior within complex biofilm communities.
Purpose of the Study:
- To investigate the association between enteric viruses and biofilms on food contact surfaces.
- To understand how biofilms influence viral adhesion to various materials.
Main Methods:
- Biofilms of mono- and multispecies cultures were formed on glass, stainless steel, and polystyrene.
- Murine norovirus, rotavirus, and hepatitis A virus were incubated with biofilms for varying durations (15 min, 90 min, 24 h).
Main Results:
- Biofilms generally influenced enteric virus adhesion to surfaces.
- Rotavirus attachment increased 7000-fold with Pseudomonas fluorescens on polystyrene after 24h.
- Hepatitis A virus showed affinity for lactic acid bacteria biofilms.
- Murine norovirus attachment was least affected by biofilms.
Conclusions:
- Biofilm presence significantly enhances enteric virus adhesion, posing potential food safety risks.
- Viral association with biofilms varies based on virus type, environmental conditions, and biofilm composition.
- Further research is needed to elucidate the mechanisms behind these virus-biofilm interactions.
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