Rhodamine-Based Fluorescent Probe for Highly Selective Determination of Hg²

The determination of mercuric ions (Hg²⁺) in environmental and biological samples has attracted the attention of researchers lately. In the present work, a novel turn-on Hg²⁺ fluorescent probe utilizing a rhodamine derivative had been constructed and prepared. The probe could highly sensitively and selectively sense Hg²⁺. In the presence of excessive Hg²⁺, the probe displayed about 52-fold fluorescence enhancement in 50% H₂O/CH₃CH₂OH (pH, 7.24). In the meantime, the colorless solution of the probe turned pink upon adding Hg²⁺. Upon adding mercuric ions, the probe interacted with Hg²⁺ and formed a 1:1 coordination complex, which had been the basis for recognizing Hg²⁺. The probe displayed reversible dual colorimetric and fluorescence sensing of Hg²⁺ because rhodamine's spirolactam ring opened upon adding Hg²⁺. The analytical performances of the probe for sensing Hg²⁺ were also studied. When the Hg²⁺ concentration was altered in the range of 8.0 × 10^-8 to 1.0 × 10^-5 mol L^-1, the fluorescence intensity showed an excellent linear correlation with Hg²⁺ concentration. A detection limit of 3.0 × 10^-8 mol L^-1 had been achieved. Moreover, Hg²⁺ in the water environment and A549 cells could be successfully sensed by the proposed probe.