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Transcriptome analysis of well-differentiated laryngeal squamous cell carcinoma cells in below-background environment
Yilin Liu1,2, Yuzhu Gao1,2, Jiahan Cheng1,3
1Deep Underground Space Medical Center, West China Hospital, Sichuan University, Chengdu, China.
Annals of Translational Medicine
|August 29, 2022
Summary
Below-background radiation (BBR) inhibits laryngeal cancer cell growth by altering gene expression. This study reveals molecular changes and signaling pathways involved in the response to BBR, offering insights into cancer cell proliferation under unique environmental conditions.
Area of Science:
- Molecular Biology
- Cancer Research
- Radiation Biology
Background:
- Preliminary studies indicated that below-background radiation (BBR) inhibits the growth of laryngeal squamous cell carcinoma cells (FD-LSC-1).
- The precise cellular responses and underlying molecular mechanisms of this growth inhibition under BBR remain largely unknown.
Purpose of the Study:
- To investigate the molecular differences in FD-LSC-1 cells exposed to BBR compared to normal radiation conditions.
- To identify transcriptional changes and signaling pathways involved in the cellular response to BBR.
Main Methods:
- Whole-transcriptome analysis of FD-LSC-1 cells grown in a deep underground laboratory (BBR) versus an above-ground laboratory (normal radiation).
- Analysis included messenger RNAs (mRNAs), long non-coding RNAs (lncRNAs), circular RNAs (circRNAs), and microRNAs (miRNAs).
- Functional enrichment analyses (Gene Ontology, KEGG pathways) and Bayesian network construction were performed on differentially expressed genes, with validation by quantitative real-time PCR.
Main Results:
- Significant differential expression was observed for 671 mRNAs, 286 lncRNAs, 489 circRNAs, and 6 miRNAs under BBR.
- Gene Ontology analysis highlighted enrichment in biological regulation, metabolic processes, response to stimulus, cell cycle, and modification processes.
- KEGG pathway analysis revealed the critical roles of TGF-β and Hippo signaling in transcriptional regulation, impacting cell proliferation genes such as SMAD, SMAD7, CDH1, EGR1, and BMP2.
Conclusions:
- Below-background radiation induces significant transcriptional alterations in laryngeal squamous cell carcinoma cells.
- The observed inhibitory effect on cell growth is linked to widespread changes in biological processes and canonical proliferation pathways, particularly TGF-β and Hippo signaling.
