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Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
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Pathway engineering facilitates efficient protein expression in Pichia pastoris.

Chao Liu1, Jin-Song Gong2, Chang Su1,3

  • 1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Lihu Avenue No. 1800, Wuxi, 214122, People's Republic of China.

Applied Microbiology and Biotechnology
|August 30, 2022
PubMed
Summary

Pichia pastoris efficiently secretes proteins using molecular chaperones and optimized pathways. This review details protein folding, modification, and transport mechanisms to improve industrial protein production.

Keywords:
ChaperonePichia pastorisProtein secretionSecretion pathway engineering

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Microbial Engineering

Background:

  • Pichia pastoris is a key host for heterologous protein production.
  • The AOX1 promoter and α-pre-pro signal sequence drive high extracellular protein expression.
  • Protein secretion involves complex cellular processes including folding, modification, and transport.

Purpose of the Study:

  • To summarize protein secretion, modification, and transportation in Pichia pastoris.
  • To elucidate the roles of key proteins in these processes.
  • To provide insights into co-expression strategies for enhanced protein production.

Main Methods:

  • Review of existing literature on Pichia pastoris protein secretion pathways.
  • Analysis of the mechanisms of molecular chaperones in protein folding.
  • Examination of protein glycosylation and vesicular transport systems.

Main Results:

  • Detailed summary of chaperone-assisted protein folding and co-expression effects.
  • Comprehensive review of protein glycosylation modifications in P. pastoris.
  • Identification of current challenges in the protein secretion pathway and proposed solutions.

Conclusions:

  • Optimizing intracellular pathways is crucial for efficient protein expression in P. pastoris.
  • Understanding chaperone functions and glycosylation is key to improving secretion.
  • This review provides a foundation for industrial-scale protein production using P. pastoris.