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A 3D bioprinted decellularized extracellular matrix/gelatin/quaternized chitosan scaffold assembling with poly(ionic
1State Key Laboratory of Fine Chemicals, Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian, 116024, China.
International Journal of Biological Macromolecules
|August 30, 2022
Summary
This study developed a novel 3D bioprinted scaffold (dGQP) using decellularized extracellular matrix and poly(ionic liquids) for advanced skin tissue engineering, demonstrating potent antibacterial and hemostatic properties.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Developing effective bioinks for 3D bioprinting skin substitutes is crucial for tissue engineering.
- Existing bioinks often struggle to mimic native skin microenvironments and prevent bacterial infections.
- A need exists for advanced scaffolds with antibacterial and hemostatic capabilities.
Purpose of the Study:
- To create and characterize a novel decellularized extracellular matrix/gel/chitosan (dGQ) scaffold incorporating poly(ionic liquids) (PILs) for 3D bioprinting (dGQP).
- To evaluate the antibacterial, hemostatic, and biocompatibility properties of the dGQP scaffold for skin tissue engineering applications.
- To assess the potential of the dGQP scaffold in mimicking the skin microenvironment and promoting tissue regeneration.
Main Methods:
- Extrusion 3D bioprinting technology was employed to fabricate the dGQP scaffold.
- The hybrid scaffold was assembled using decellularized extracellular matrix (dECM), gelatin (Gel), chitosan (QCS), and poly(ionic liquids) (PILs).
- Morphological, mechanical, porosity, hydrophilicity, biodegradation, hemostatic, antibacterial, and biocompatibility properties were systematically evaluated.
Main Results:
- The dGQP scaffold demonstrated rapid and sustained release of PILs, achieving nearly 100% antibacterial activity against E. coli and S. aureus for up to 7 days.
- The scaffold exhibited excellent hemostatic properties and hemocompatibility, with no significant hemolysis observed.
- In vitro studies showed initial slow cell growth on the dGQP scaffold, followed by rapid proliferation and high extracellular matrix (ECM) secretion by day 7.
Conclusions:
- The developed dGQP scaffold possesses significant antibacterial and hemostatic capabilities, making it a promising candidate for skin tissue engineering.
- The scaffold effectively supports cell proliferation and ECM production, indicating its potential to mimic native skin microenvironments.
- This advanced 3D bioprinted scaffold holds considerable potential for clinical applications in skin regeneration and wound healing.
