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Proteomic Profiling of Macrophages by 2D Electrophoresis
Published on: November 4, 2014
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Dielectrophoretic characterization of macrophage phenotypes
Mi Thant Mon Soe1, Kara L Spiller2, Moses Noh1
1Mechanical Engineering and Mechanics, Drexel University, Philadelphia, Pennsylvania, USA.
Electrophoresis
|September 2, 2022
Summary
This study shows that dielectrophoresis (DEP) can distinguish between human monocyte and macrophage phenotypes (M0, M1, M2a). M1 macrophages exhibit a unique DEP response, indicating altered bioelectric properties.
Area of Science:
- Cellular and Molecular Biology
- Biophysics
- Immunology
Background:
- Macrophage phenotypes (M0, M1, M2a) are crucial in various biological processes and diseases.
- Dielectrophoresis (DEP) is a technique sensitive to cell biophysical properties.
Purpose of the Study:
- To characterize the dielectrophoretic (DEP) responses of human monocytes and distinct macrophage phenotypes (M0, M1, M2a).
- To investigate how changes in macrophage phenotype influence their bioelectric properties.
Main Methods:
- Human THP1 monocytes and differentiated M0, M1, and M2a macrophages were analyzed using DEP.
- Crossover frequencies were measured using interdigitated electrodes in a novel low-conductivity media.
- Membrane capacitances were calculated from DEP data.
Main Results:
- Human monocytes exhibited a distinguishable DEP response compared to mature macrophages.
- M1 macrophages showed a distinct DEP response from M0 and M2a macrophages.
- Median crossover frequencies (kHz) were 38 (monocytes), 21 (M0), 11 (M1), and 23 (M2a).
- Calculated membrane capacitances (F/m²) were 0.0111 (monocytes), 0.0128 (M0), 0.0244 (M1), and 0.0117 (M2a).
Conclusions:
- DEP successfully differentiates human monocytes from macrophages and distinguishes the pro-inflammatory M1 phenotype.
- Dielectrophoretic responses and calculated membrane capacitances reflect alterations in macrophage phenotype-specific bioelectric properties.
