Isobaric labeling: Expanding the breadth, accuracy, depth, and diversity of sample multiplexing

Area of Science:

• Proteomics
• Analytical Chemistry
• Biochemistry

Background:

• Isobaric labeling is a primary method for proteome-wide abundance measurements.
• It enables multiplexing for analyzing proteins and posttranslational modifications across samples.

Purpose of the Study:

• To highlight advancements in isobaric labeling for proteomics.
• To discuss expanding multiplexing capabilities, improving quantitative accuracy, and increasing proteome depth.

Main Methods:

• Mass spectrometry coupled with isobaric labeling techniques.
• Development of quality control standards and advanced data acquisition methods.

Main Results:

• Isobaric labeling offers unparalleled capabilities for sample multiplexing.
• Quality control standards and evolving data acquisition methods enhance quantitative accuracy and reproducibility.

Conclusions:

• Standardized methodologies are crucial for high-throughput proteomics workflows.
• Future developments will further solidify isobaric labeling's role in comprehensive proteome profiling.