One step cascade detection of galactose based on a galactose oxidase-composited peroxidase nanozyme

Abnormal galactose metabolism is the main cause of galactosemia, which makes the accurate and rapid analysis of galactose levels in food and organism the key issue at present. In this study, a novel strategy for one-step galactose determination was proposed based on galactose oxidase and copper-based metal-organic framework complexes (GAOx@MOF) with dual catalytic activities at neutral pH. Typically, GAOx catalyzes the oxidation of the C6 hydroxyl group of D-galactose to generate an aldehyde (D-galactose-hexanedial), and coupled with the reduction of dioxygen to H₂O₂, which was immediately transformed to ˙OH by mimicking peroxidase activity and at the same time oxidized ABTS to a green product with a clear colorimetric signal. The whole process was completed using one buffer, which simplified the procedure and increased the sensitivity. Moreover, the proposed method can also be used for the quantitative analysis of galactose. It showed a good linear relationship at 20-1000 μM, while the LOD was 6.67 μM. Furthermore, the strategy has been successfully utilized for galactose determination in milk samples, which proved its promising applications in clinical analysis and the food industry.