Comparison of UVC Sensitivity and Dectin-2 Expression Between Malignant and Non-malignant Cells
Ayaka Shindo1, Midori Kusano1, Hiroshi Sakagami2
1Division of Feeding and Swallowing Rehabilitation, Department of Restorative and Biomaterials Sciences, Meikai University School of Dentistry, Saitama, Japan.
In Vivo (Athens, Greece)
|September 13, 2022
Summary
Malignant cells are more sensitive to ultraviolet C (UVC) sterilization than normal cells. Sodium ascorbate protects against UVC cytotoxicity, and Dectin-2 expression may not determine UVC sensitivity.
Area of Science:
- Cell biology
- Photobiology
- Cancer research
Background:
- The COVID-19 pandemic highlighted the importance of ultraviolet C (UVC) sterilization in shared environments.
- Understanding UVC sensitivity across different cell types is crucial for optimizing sterilization protocols.
Purpose of the Study:
- To assess the UVC sensitivity of 18 malignant and nonmalignant cell lines.
- To investigate the protective effects of sodium ascorbate against UVC radiation.
- To determine the role of Dectin-2 in UVC sensitivity.
Main Methods:
- Cell viability was measured using the MTT assay after UVC exposure.
- Anti-UV activity was quantified by comparing cytotoxic and effective concentrations.
- Dectin-2 expression was analyzed via flow cytometry.
Main Results:
- Oral squamous cell carcinoma, neuroblastoma, glioblastoma, leukemia, and melanoma cells exhibited higher UVC sensitivity than normal fibroblasts.
- Normal oral and dermal fibroblasts demonstrated resistance to UVC.
- Sodium ascorbate effectively neutralized UVC cytotoxicity at micromolar concentrations.
Conclusions:
- Malignant cells generally show greater UVC sensitivity than their normal counterparts due to higher proliferation rates.
- Dectin-2 protein expression alone does not appear to be a definitive factor in UVC sensitivity.
