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Updated: Aug 28, 2025

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
Published on: May 8, 2015
Purification of Self-Assembled DNA Tetrahedra Using Gel Electrophoresis
Akul Patel1, Vibhav Valsangkar1, Ken Halvorsen1
1The RNA Institute, University at Albany, State University of New York, New York.
Abstract:
DNA nanostructures have found applications in a variety of fields such as biosensing, drug delivery, cellular imaging, and computation. Several of these applications require purification of the DNA nanostructures once they are assembled. Gel electrophoresis-based purification of DNA nanostructures is one of the methods used for this purpose. Here, we describe a step-by-step protocol for a gel-based method to purify self-assembled DNA tetrahedra. With further optimization, this method could also be adapted for other DNA nanostructures. © 2022 Wiley Periodicals LLC. Basic Protocol: Purification of self-assembled DNA tetrahedra.
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