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UGT88B2: A promiscuous O-glycosyltransferase from Carthamus tinctorius
Song-Yang Sui1, Rui-Mingqian Guo1, Ke-Bo Xie2
1College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China.
Objective:
In order to obtain new glycosyltransferases with highly efficient catalysis, the glycosyltransferases from Carthamus tinctorius which contains diverse types of glycosides were mined. Methods: A new glycosyltransferase gene (UGT88B2) with full length was obtained by PCR and further transformed into Escherichia coli for heterologous expression. The catalytic activity of recombinant UGT88B2 was determined by HPLC-MSn. The structures of representative catalytic products were elucidated by MS and NMR.
Results:
UGT88B2 exhibited catalytic promiscuity and various patterns in glycosylation of flavonoids with high efficiency.
Conclusion:
A new glycosyltransferase named UGT88B2 was successfully mined and can be employed as enzymatic tools in glycosylation of flavonoids.
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