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Updated: Aug 28, 2025

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
Published on: November 16, 2019
Optimized ultrahigh field parallel transmission workflow using rapid presaturated TurboFLASH transmit field mapping
Dario Bosch1,2, Jonas Bause1, Ole Geldschläger1
1High-Field MR Center, Max Planck Institute for Biological Cybernetics, Tübingen, Germany.
Purpose:
To evaluate the usage of three-dimensional (3D) presaturated TurboFLASH (satTFL) for and mapping on single channel and parallel transmission (pTx) systems.
Methods:
maps recorded with 3D satTFL were compared to maps from three other 3D mapping sequences in an agar phantom. Furthermore, individual-channel maps of 18 human subjects were recorded with 3D satTFL using interferometry. A neural network was trained for masking of the maps.
Results:
Out of the sequences compared satTFL was the only one with a mapping range exceeding well over 90°. In regions with lower flip angles there was high correspondence between satTFL and AFI. DREAM and double angle method also showed high qualitative similarity, however the magnitude differed from the other two measurements. The individual-channel maps were successfully used for pTx pulse calculation in a separate study.
Conclusion:
3D satTFL can record high-quality maps with a high dynamic range in a short time. Correspondence with AFI maps is high, while measurement duration is reduced drastically.

