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Related Experiment Video

Updated: Aug 28, 2025

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

QUADRatlas: the RNA G-quadruplex and RG4-binding proteins database.

Sébastien Bourdon^1,2,3, Pauline Herviou^2,3, Leïla Dumas^2,3

¹Laboratory of RNA Regulatory Networks, Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, 38123 Trento, Italy.

Nucleic Acids Research

|September 20, 2022

Summary

QUADRatlas is a new database for RNA G-quadruplexes (RG4s), crucial regulators of gene expression. It integrates experimental and predicted RG4 data with RNA-binding protein interactions to explore RG4 biology and disease links.

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Last Updated: Aug 28, 2025

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Area of Science:

Genomics
Molecular Biology
Bioinformatics

Background:

RNA G-quadruplexes (RG4s) are non-canonical structures regulating gene expression.
RG4 functions are modulated by RNA-binding proteins (RBPs).
Understanding RG4-RBP interactions is key for disease research and therapeutics.

Purpose of the Study:

To present QUADRatlas, a comprehensive database of human RG4s.
To integrate RG4 data with RBP interactions and disease associations.
To provide tools for analyzing RG4 formation and RBP binding.

Main Methods:

Curated experimentally-derived RG4 data.
Computational prediction of RG4s.
Mining known RG4-RBP interactions and RBP binding sites.
Development of analysis and visualization tools.

Main Results:

QUADRatlas database containing experimentally-derived and predicted RG4s.
Enrichment of RG4 data with biological function and disease associations.
Integration of RG4s with a large dataset of RBP binding sites.
Availability of tools for RG4 prediction and RBP binding analysis.

Conclusions:

QUADRatlas facilitates transcriptome-wide exploration of RG4s and their RBP interactions.
The database enables hypothesis generation regarding RG4 regulation, function, and pathogenicity.
QUADRatlas advances the understanding of RG4 biology and its role in disease.