Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two distinct sequence elements mediate retroviral gene expression in embryonal carcinoma cells.

H Weiher, E Barklis, W Ostertag

    Journal of Virology
    |September 1, 1987
    PubMed
    Summary

    Moloney murine leukemia virus (M-MuLV) gene expression is blocked in early embryos but can be restored in embryonal carcinoma cells. Two specific DNA sequence elements are critical for enabling M-MuLV gene expression in these cells.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    MeCP2-regulated miRNAs control early human neurogenesis through differential effects on ERK and AKT signaling.

    Molecular psychiatry·2017
    Same author

    Tumor suppressor functions of Dnmt3a and Dnmt3b in the prevention of malignant mouse lymphopoiesis.

    Leukemia·2013
    Same author

    Gene targeting in human pluripotent cells.

    Cold Spring Harbor symposia on quantitative biology·2011
    Same author

    Epigenetic memory in induced pluripotent stem cells.

    Nature·2010
    Same author

    Uber infektiöse Substrukturen aus Bakteriophagen. VI. A simple method for separating the replicative form from single-stranded phiX174 DNA.

    Journal of molecular biology·2009
    Same author

    Uber infektiöse Substrukturen aus Escherichia coli Bakteriophagen. VIII. On the tertiary structure and biological properties of phiX174 replicative form.

    Journal of molecular biology·2009

    Area of Science:

    • Retroviral gene expression
    • Developmental biology
    • Molecular genetics

    Background:

    • Moloney murine leukemia virus (M-MuLV) and its derived vectors show limited expression in early mouse embryos and embryonal carcinoma (EC) cells.
    • M-MuLV-derived mutants can confer drug resistance to EC cells, indicating potential for gene transfer.

    Purpose of the Study:

    • To identify the specific DNA sequences responsible for M-MuLV gene expression in F9 and PCC4 embryonal carcinoma cell lines.
    • To elucidate the regulatory mechanisms governing retroviral gene expression in early developmental contexts.

    Main Methods:

    • Analysis of M-MuLV gene expression in F9 and PCC4 EC cells.
    • Identification and characterization of critical sequence elements within the M-MuLV genome.
    • Mutation analysis to assess the impact of specific sequences on viral RNA levels.

    Related Experiment Videos

    Main Results:

    • Two distinct sequence elements synergistically enhance M-MuLV expression in EC cells.
    • One element is located in the U3 region of the viral long terminal repeat (LTR).
    • A second element in the 5' untranslated region, affected by a single point mutation, influences stable RNA levels.

    Conclusions:

    • Specific sequence elements in the M-MuLV LTR and 5' UTR are essential for gene expression in embryonal carcinoma cells.
    • The identified regulatory mechanism may share similarities with human immunodeficiency virus (HIV) regulation in T cells.
    • These findings provide insights into the control of retroviral gene expression during early development.