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Updated: Aug 28, 2025

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors
Published on: July 16, 2012
[Genotypic recombination and hepatitis C virus]
F Legrand-Abravanel1, J Izopet1
1Inserm U563, Centre de physiopathologie, Toulouse-Purpan, 31300 Toulouse, CHU, Hôpital Purpan, Laboratoire de virologie, Institut fédératif de biologie, 31300 Toulouse-Purpan.
Abstract:
Hepatitis C virus (HCV) is an RNA virus, which belongs to the genus Hepacivirus within the Flaviviridae family. Recombinant viruses have been described in the three genera of this family. HCV is a highly variable virus, whose strains are classified among six genotypes. The first intergenotypic recombinant HCV 2k/1b was identified in 2002 in Saint Petersburg. To date a limited number of intragenotypic or intergenotypic recombinant strains were characterized in vivo. The crossover point of the recombinant strains was mainly found at the NS2-NS3 junction. Regarding the small number of recombinant strains described in the literature, the phenomenon of recombination might be rare. However, its prevalence may be underestimated since these viruses are not routinely searched. The impact of recombination on HCV epidemiology or physiopathology remains to be elucidated.
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