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Hydroxycinnamic Acid Derivatives from Coffee Extracts Prevent Amyloid Transformation of Alpha-Synuclein
Maria Medvedeva1, Natalia Kitsilovskaya2,3, Yulia Stroylova4
1Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, 119234 Moscow, Russia.
Biomedicines
|September 23, 2022
Summary
Coffee extracts, rich in 3,4-dimethoxycinnamic acid, inhibit alpha-synuclein amyloid aggregation. This suggests coffee may help prevent synucleinopathies like Parkinson's disease.
Area of Science:
- Biochemistry
- Neuroscience
- Pharmacology
Background:
- Hydroxycinnamic acid derivatives are known to inhibit amyloid transformation of alpha-synuclein and prion proteins.
- Alpha-synuclein aggregation is a key pathological hallmark of synucleinopathies, including Parkinson's disease.
Purpose of the Study:
- To quantify 3-hydroxycinnamic acid derivatives in coffee extracts.
- To evaluate the inhibitory activity of these derivatives against alpha-synuclein amyloid aggregation.
Main Methods:
- Quantitative mass spectrometry was used to identify and quantify hydroxycinnamic acid derivatives in coffee extracts.
- Thioflavin T assay was employed to determine the inhibitory concentrations and constants of alpha-synuclein fibrillization.
Main Results:
- Significant levels of 3,4-dimethoxycinnamic acid were detected in coffee extracts, with ferulic acid present in trace amounts.
- Roasted coffee extracts also contained 3-methoxy-4-acetamidoxycinnamic acid.
- Both black and green coffee extracts demonstrated dose-dependent inhibition of alpha-synuclein fibrillization, with green coffee showing comparable or greater efficacy.
Conclusions:
- Coffee extracts, particularly due to 3,4-dimethoxycinnamic acid, effectively prevent alpha-synuclein pathological transformation in vitro.
- Coffee consumption may offer a preventative strategy for synucleinopathies such as Parkinson's disease.
