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Updated: Aug 27, 2025

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Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
Published on: June 9, 2017
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Assay of GTPγS Binding in Autoradiography
Marina Gabaglio1, Pamela Prini1, Erica Zamberletti1
1Department of Biotechnology and Life Sciences, University of Insubria, Busto Arsizio, (VA), Italy.
Methods in Molecular Biology (Clifton, N.J.)
|September 24, 2022
Abstract:
Autoradiography of radiolabeled GTPγS ([35S]GTPγS) binding is a relevant technique to study the function of G protein-coupled receptors (GPCRs) ex vivo. Here, we describe the protocol for such a method, suitable for investigating CB1 receptor functionality in tissue slices from rodent brains.

