Related Experiment Video
Updated: Aug 27, 2025

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
Published on: September 7, 2022
Impact of temperature on optical sensing in biology based on investigation of SARS-CoV-2
Małgorzata Szczerska1, Paweł Wityk2, Paulina Listewnik1
1Department of Metrology and Optoelectronics, Faculty of Electronics, Telecommunications and Informatics, Gdańsk University of Technology, Gdańsk, Poland.
Abstract:
In this paper, we present an investigation of the influence of the temperature on the sensing of biological samples. We used biofunctionalized microsphere-based fiber-optic sensor to detect immunoglobulin G attached to the sensor head at temperatures relevant in biological research: 5°C, 25°C, and 55°C. The construction of the sensor allowed us to perform measurements in the small amount of solution. The results of our experiment confirm substantial changes in the measured reflected optical power, indicating the need to control the temperature during such measurements. The sensitivity of the sensor used in this research is 8.82 nW/°C. Coefficient R was also calculated and it equals 0.998, which shows good fit between theoretical linear fit and obtained measured data.
Related Concept Videos
Physical Methods for Controlling Microbial Growth: Temperature
Effect of Temperature Change on Reaction Rate
Factors Influencing Microbial Growth: Temperature
Effects of Temperature on Free Energy
Factors Affecting Body Temperature
Factors may include:
Atomic Spectroscopy: Effects of Temperature
At thermal equilibrium, the relative populations of excited and ground state atoms can be estimated using the Maxwell–Boltzmann distribution. For example, an increase in temperature...

