Robotic APTamer-Enabled Electrochemical Reader (RAPTER) System for Automated Aptamer-Mediated Electrochemical...

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Robotics

Background:

• Electrochemical aptamer-based (E-AB) sensors offer sensitive, reagent-less, and cost-effective molecular detection.
• Advances enable continuous drug monitoring and automated hormone analysis in biological systems.

Purpose of the Study:

• To detail the methodology for an automated E-AB conformational change-based robotic sensing platform.
• To demonstrate the adaptability of this platform for diverse experimental needs.

Main Methods:

• Utilized an open-source programmable robotic system.
• Integrated conformational change-induced electron transfer kinetics for sensing.
• Developed an automated platform for E-AB sensor operation.

Main Results:

• Established a methodology for an automated robotic sensing platform.
• The platform is adaptable to a wide range of experimental scenarios.
• Demonstrated the potential for sensitive and reagent-less molecular sensing.

Conclusions:

• An automated robotic sensing platform for E-AB sensors has been developed.
• This system offers a versatile and cost-effective solution for molecular sensing.
• The platform facilitates advanced applications such as pharmacokinetic monitoring and hormone analysis.