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Updated: Aug 27, 2025

In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts

In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts

Published on: August 14, 2019

How to Generate a Vascular-Labelled Transgenic Zebrafish Model to Study Tumor Angiogenesis and Extravasation.

Roxana E Oberkersch¹, Jacopo Lidonnici¹, Massimo M Santoro²

¹Laboratory of Angiogenesis and Cancer Metabolism, Department of Biology, University of Padova, Padova, Italy.

Methods in Molecular Biology (Clifton, N.J.)

|September 26, 2022

Summary

Transgenic zebrafish are a valuable tool for studying blood vessel formation (angiogenesis) and tumor growth. This research details methods for creating these zebrafish models to observe tumor development and blood vessel interactions in vivo.

Keywords:

Endothelial cells Extravasation Mural cells Tumor angiogenesis Zebrafish embryos

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Last Updated: Aug 27, 2025

In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts

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Published on: August 14, 2019

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Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Generation of Zebrafish Larval Xenografts and Tumor Behavior Analysis

Published on: June 19, 2021

Area of Science:

Developmental Biology
Genetics
Cancer Research

Background:

Transgenic animal models are essential in modern biological research.
Zebrafish are highly effective for transgenesis due to their prolific egg production and rapid generation time.
Zebrafish facilitate in vivo studies of vertebrate embryonic vasculature, particularly in angiogenesis research.

Purpose of the Study:

To describe a common technique for generating vascular-labeled transgenic zebrafish embryos.
To highlight the applications of these zebrafish models in studying tumor angiogenesis.
To demonstrate the visualization of tumor extravasation using this transgenic system.

Main Methods:

Generation of transgenic zebrafish embryos with specific vascular labeling.
Utilizing zebrafish transgenesis technology for in vivo observation.
Coupling transgenesis with angiogenesis studies for detailed vascular analysis.

Main Results:

Successful generation of vascular-labeled transgenic zebrafish embryos.
Demonstration of the utility of these models for observing tumor angiogenesis.
Visualization of tumor cell extravasation within the embryonic vasculature.

Conclusions:

Transgenic zebrafish provide a powerful and accessible system for studying angiogenesis.
This methodology enables detailed in vivo investigation of tumor vascularization and metastasis.
Zebrafish models are crucial for advancing our understanding of tumor angiogenesis and extravasation.