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Updated: Aug 27, 2025

Hyperpolarized 13C Metabolic Magnetic Resonance Spectroscopy and Imaging
Published on: December 30, 2016
Nuclear Overhauser spectroscopy in hyperpolarized water - chemical vs. magnetic exchange
Ludovica Martina Epasto1,2, Philipp Honegger3,4, Kateryna Che1
1University of Vienna, Faculty of Chemistry, Department of Biological Chemistry, Währingerstr. 38, 1090 Vienna, Austria. dennis.kurzbach@univie.ac.at.
Abstract:
Dissolution dynamic nuclear polarization (dDNP) is a versatile hyperpolarization technique to boost signal intensities in nuclear magnetic resonance (NMR) spectroscopy. The possibility to dissolve biomolecules in a hyperpolarized aqueous buffer under mild conditions has recently widened the scope of NMR by dDNP. The water-to-target hyperpolarization transfer mechanisms remain yet unclear, not least due to an often-encountered dilemma of dDNP experiments: The strongly enhanced signal intensities are accompanied by limited structural information as data acquisition is restricted to short time series of only one-dimensional spectra or a single correlation spectrum. Tackling this challenge, we combine dDNP with molecular dynamics (MD) simulations and predictions of cross-relaxation rates to unravel the spin dynamics of magnetization flow in hyperpolarized solutions.
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