Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

3.1K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
3.1K
Structure of Cadherins01:25

Structure of Cadherins

3.5K
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins”   is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
3.5K
Adherens Junctions01:24

Adherens Junctions

5.0K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types –  adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as  epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as  heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
5.0K
Tension Response at Adherens Junctions01:26

Tension Response at Adherens Junctions

2.8K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
2.8K
Catenins01:23

Catenins

2.4K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
2.4K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.7K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The V-ATPase/ATG16L1 axis drives membrane remodeling during epithelial morphogenesis.

Nature communications·2026
Same author

Defective mitochondria remodelling in B cells leads to an aged immune response.

Nature communications·2024
Same author

[Ethical respect of weakness].

Cuadernos de bioetica : revista oficial de la Asociacion Espanola de Bioetica y Etica Medica·2022
Same author

Multiple ciliary localization signals control INPP5E ciliary targeting.

eLife·2022
Same author

Deciphering the interplay between autophagy and polarity in epithelial tubulogenesis.

Seminars in cell & developmental biology·2022
Same author

Actomyosin fibers DApPLE epithelial apical junctions.

The Journal of cell biology·2022
Same journal

Chromosome condensation mechanically primes the nucleus for mitosis.

The EMBO journal·2026
Same journal

NDR kinase SAX-1 controls dendrite branch-specific elimination during neuronal remodeling in C. elegans.

The EMBO journal·2026
Same journal

Assembly of the catalytic module and the rotor of human ATP synthase.

The EMBO journal·2026
Same journal

Substrate-induced assembly and functional mechanism of the membrane protein insertase SecYEG-YidC.

The EMBO journal·2026
Same journal

Conformational changes of the baseplate regulating tail contraction of Staphylococcus phage 812.

The EMBO journal·2026
Same journal

Cellular assembly and functional resilience of the mammalian RNA exosome.

The EMBO journal·2026
See all related articles

Related Experiment Video

Updated: Aug 26, 2025

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
08:15

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

10.6K

Cadherin-mediated adhesion takes control.

Gonzalo Herranz1, Fernando Martín-Belmonte1

  • 1Program of Tissue and Organ Homeostasis, Centro de Biología Molecular "Severo Ochoa", CSIC-UAM, Madrid, Spain.

The EMBO Journal
|October 4, 2022
PubMed
Summary
This summary is machine-generated.

The formation of the apical membrane initiation site (AMIS) is crucial for epithelial cell polarization. A new study shows that cadherin-mediated cell adhesion is essential for correctly localizing the AMIS.

More Related Videos

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

16.2K
Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
13:22

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

Published on: November 2, 2011

15.1K

Related Experiment Videos

Last Updated: Aug 26, 2025

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules
08:15

Bead Aggregation Assays for the Characterization of Putative Cell Adhesion Molecules

Published on: October 17, 2014

10.6K
Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

16.2K
Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
13:22

Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface

Published on: November 2, 2011

15.1K

Area of Science:

  • Cell Biology
  • Epithelial Biology
  • Molecular Cell Biology

Background:

  • Epithelial cell polarization is fundamental for tissue function.
  • The apical membrane initiation site (AMIS) is a critical structure in establishing cell polarity.
  • Understanding AMIS formation mechanisms is key to deciphering epithelial development and disease.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying the localization of the apical membrane initiation site (AMIS).
  • To determine the role of cell adhesion molecules in the establishment of epithelial polarity.

Main Methods:

  • Utilized advanced microscopy techniques to visualize AMIS formation in epithelial cells.
  • Employed genetic and biochemical approaches to study the function of cadherins in polarity establishment.

Main Results:

  • Demonstrated that cadherin-mediated cell adhesion is a prerequisite for the proper localization of the AMIS.
  • Identified specific cadherin interactions that regulate AMIS positioning.
  • Showcased the dynamic interplay between cell adhesion and polarity pathways.

Conclusions:

  • Cadherin-mediated adhesion plays a pivotal role in organizing the apical domain through AMIS localization.
  • This finding provides new insights into the regulation of epithelial cell polarity.
  • Highlights potential therapeutic targets for diseases involving disrupted epithelial organization.