Related Experiment Video
Updated: Aug 26, 2025

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
Published on: February 3, 2021
Fluorometer Control and Readout Using an Arduino Nano 33 BLE Sense Board
Zechariah B Kitzhaber1, Caitlyn M English1, Kazi Ragib I Sanim2
1Department of Chemistry and Biochemistry, 2629University of South Carolina, Columbia, SC, USA.
Abstract:
We describe the control and interfacing of a fluorometer designed for aerial drone-based measurements of chlorophyll-a using an Arduino Nano 33 BLE Sense board. This 64 MHz controller board provided suitable resolution and speed for analog-to-digital (ADC) conversion, processed data, handled communications via the Robot Operating System (ROS) and included a variety of built-in sensors that were used to monitor the fluorometer for vibration, acoustic noise, water leaks and overheating. The fluorometer was integrated into a small Uncrewed Aircraft System (sUAS) for automated water sampling through a Raspberry Pi master computer using the ROS. The average power consumption was 1.1 W. A signal standard deviation of 334 µV was achieved for the fluorescence blank measurement, mainly determined by the input noise equivalent power of the transimpedance amplifier. An ADC precision of 130 µV for 10 Hz chopped measurements was achieved for signals in the input range 0-600 mV.

