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Solid-state NMR methods for the characterization of bioconjugations and protein-material interactions
Linda Cerofolini1, Giacomo Parigi2, Enrico Ravera3
1Consorzio Interuniversitario Risonanze Magnetiche di Metalloproteine, Via Luigi Sacconi 6, 50019, Sesto Fiorentino, Italy.
Solid State Nuclear Magnetic Resonance
|October 14, 2022
Summary
Solid-state NMR of proteins has advanced significantly, enabling complex applications. This review details protein solid-state NMR for bioconjugation, protein chemistry, and functional biomaterials.
Area of Science:
- Biophysical Chemistry
- Materials Science
Background:
- Protein solid-state NMR has undergone substantial advancements in hardware and sample preparation over the past 20 years.
- These developments have expanded the technique's capabilities for analyzing complex biological systems.
Approach:
- This review provides an account of protein solid-state NMR focusing on its applications.
- The discussion covers advancements in instrumentation and methodologies.
- Emphasis is placed on the technique's utility in specialized areas.
Key Points:
- Solid-state NMR is now suitable for intricate applications such as bioconjugation.
- The technique is valuable for detailed protein chemistry investigations.
- Applications extend to the development of functional biomaterials.
Conclusions:
- Protein solid-state NMR is a powerful technique with a growing range of applications.
- The continuous evolution of hardware and sample preparation fuels its utility in complex biological and material science fields.
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