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Heat inactivation of monkeypox virus
Christophe Batéjat1, Quentin Grassin1, Maxence Feher1
1Institut Pasteur, Université Paris Cité, Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence (CIBU), F-75015 Paris, France.
Journal of Biosafety and Biosecurity
|October 17, 2022
Summary
Monkeypox virus (MPXV) can be inactivated quickly by heat. MPXV was inactivated in under 5 minutes at 70°C and 15 minutes at 60°C, ensuring laboratory safety.
Area of Science:
- Virology
- Biosafety
- Infectious Diseases
Background:
- Monkeypox virus (MPXV) poses a significant public health risk.
- Effective inactivation methods are crucial for laboratory safety and research continuity.
- Understanding MPXV thermal stability is essential for developing safe handling protocols.
Purpose of the Study:
- To determine the thermal inactivation kinetics of monkeypox virus (MPXV).
- To establish optimal temperature and time parameters for MPXV heat inactivation.
- To provide data for enhancing biosafety measures in laboratories handling MPXV.
Main Methods:
- Exposure of MPXV-spiked media to controlled temperatures (e.g., 60°C, 70°C).
- Incubation for defined time intervals.
- Assessment of viral infectivity post-heat treatment.
Main Results:
- MPXV was inactivated in less than 5 minutes at 70°C.
- Complete inactivation of MPXV was achieved in less than 15 minutes at 60°C.
- Thermal inactivation efficacy was consistent across different MPXV clades (West African and Central African).
Conclusions:
- Heat treatment is a highly effective method for inactivating monkeypox virus.
- Specific temperature and time parameters (e.g., 70°C for <5 min, 60°C for <15 min) ensure rapid MPXV inactivation.
- These findings support the optimization of laboratory protocols for safe MPXV manipulation and research.
