Related Experiment Video
Updated: Aug 25, 2025
11:32
A Hydrogen-Deuterium Exchange Mass Spectrometry HDX-MS Platform for Investigating Peptide Biosynthetic Enzymes
Published on: May 4, 2020
8.2K
Fundamentals of HDX-MS
Vanesa Vinciauskaite1, Glenn R Masson1
1Division of Cellular and Systems Medicine, School of Medicine, University of Dundee, Dundee, DD1 9SY, United Kingdom.
Essays in Biochemistry
|October 17, 2022
Summary
Hydrogen deuterium exchange mass spectrometry (HDX-MS) offers a versatile method for studying protein structure and dynamics. Its automation and applicability to challenging proteins make it valuable for molecular biologists.
Area of Science:
- Biochemistry
- Structural Biology
- Analytical Chemistry
Background:
- Hydrogen deuterium exchange mass spectrometry (HDX-MS) is increasingly adopted by molecular biologists for protein analysis.
- Its versatility allows investigation of proteins difficult to study with X-ray crystallography or cryo-electron microscopy.
Approach:
- This review details the fundamental principles of solvent exchange in HDX-MS.
- It outlines the standard experimental workflow for HDX-MS.
- Emerging experimental advancements and future directions in the field are highlighted.
Key Points:
- HDX-MS is valuable for determining protein structure and dynamics.
- Automation enhances the accessibility and application of HDX-MS.
- The technique complements traditional structural biology methods.
Conclusions:
- HDX-MS is a powerful and evolving technique for protein research.
- Understanding HDX-MS principles and advancements aids researchers in its application.
- The review serves as a guide for scientists interested in utilizing HDX-MS.
Related Concept Videos
01:15High-Resolution Mass Spectrometry (HRMS)
1.5K
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For...
1.5K
01:182D NMR: Overview of Heteronuclear Correlation Techniques
279
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
279
01:192D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
852
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
852
01:21Tandem Mass Spectrometry
1.2K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and signal-to-noise ratio for the analyte. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called collision-induced...
1.2K
01:21Mass Spectrometry: Complex Analysis
870
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
870
01:19Mass Spectrometry: Overview
5.6K
Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electrospray ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave...
5.6K