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Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
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Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
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In size-exclusion chromatography (SEC), also known as molecular-exclusion or gel-permeation chromatography, molecules are separated based on their sizes. This technique is important for separating large molecules such as polymers and biomolecules. The two classes of micron-sized stationary phases encountered in SEC are silica particles and cross-linked polymer resin beads. Both materials are porous, but their pore sizes vary significantly.
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Arsenosugar extracted from algae: Isolation by anionic exchange solid-phase extraction.

Alba Morales-Rodríguez1, Miquel Pérez-López1, Elle Puigpelat1

  • 1Departament d'Enginyeria Química i Química Analítica, Universitat de Barcelona, Martí i Franquès, 1-11, 08028 Barcelona, Spain; Departament de Nutrició, Ciències de l'Alimentació i Gastronomia, Campus de l'Alimentació de Torribera, Universitat de Barcelona Avda. Prat de la Riba, 171, 08921 Sta. Coloma de Gramenet, Barcelona, Spain.

Journal of Chromatography. A
|October 17, 2022
PubMed
Summary
This summary is machine-generated.

This study explores using anion exchange solid-phase extraction (SPE) cartridges for isolating arsenosugars in algae. The method offers a quicker alternative for analyzing arsenic speciation, crucial for dietary exposure assessments.

Keywords:
AlgaeArsenosugarsIC-ICP-MSstrong anion exchange-SPE

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Marine Biology

Background:

  • Arsenosugars are key arsenic species in algae, but their analysis is limited by lack of standards and complex synthesis.
  • Accurate speciation data is vital for dietary exposure assessment and understanding arsenic biochemistry.

Purpose of the Study:

  • To evaluate the effectiveness of anion exchange solid-phase extraction (SPE) cartridges for arsenosugar isolation and preconcentration.
  • To compare strong (SAX) and weak (WAX) anion exchange SPE cartridges for arsenosugar separation.

Main Methods:

  • Investigated two commercial silica-based SPE cartridges: DSC-SAX and DSC-NH2.
  • Optimized SPE parameters including pH, ionic strength, salt type, and elution solvent.
  • Analyzed eluted arsenic species using Inductively Coupled Plasma Mass Spectrometry (ICP-MS) and Ion Chromatography ICP-MS (IC-ICP-MS).

Main Results:

  • Developed an SPE procedure to isolate three arsenosugars (PO4-Sug, SO3-Sug, SO4-Sug) from other arsenic species.
  • Achieved over 75% recovery for SO3-Sug and SO4-Sug, with approximately 45% recovery for PO4-Sug.
  • Demonstrated the feasibility of SPE as a simplified method for arsenosugar analysis.

Conclusions:

  • Anion exchange SPE offers a practical and rapid approach for arsenosugar isolation and preconcentration.
  • This method aids in obtaining reliable speciation data for dietary exposure and arsenic biochemistry studies in algae.
  • Further optimization may be needed to improve recovery rates for specific arsenosugar compounds.