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High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
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Focus quality in raster-scan imaging via a multimode fiber.

Zhouping Lyu, Gerwin Osnabrugge, Pepijn W H Pinkse

    Applied Optics
    |October 18, 2022
    PubMed
    Summary

    Wavefront shaping (WFS) in multimode fiber (MMF) imaging is affected by input light position. Center input optimizes focus contrast, while edge input reduces aberrations, aiding high-field-of-view imaging.

    Area of Science:

    • Optics and Photonics
    • Biomedical Imaging
    • Fiber Optics

    Background:

    • Multimode fibers (MMF) are used as minimally invasive imaging probes.
    • Raster-scan imaging with MMF relies on wavefront shaping (WFS) to optimize foci at the fiber output.
    • Real-world MMF applications often involve partial mode control and non-ideal input light conditions.

    Purpose of the Study:

    • To investigate the impact of input light position on WFS performance in a round-core MMF.
    • To analyze the trade-offs between focus shape and contrast influenced by input position.
    • To provide insights for optimizing MMF-based raster-scan microscopy.

    Main Methods:

    • Utilized wavefront shaping (WFS) techniques to control light propagation through a round-core MMF.

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  • Varied the input light position (center vs. edge) on the fiber facet.
  • Analyzed the characteristics of the generated foci at the MMF output, including shape and contrast.

    • Main Results:

    • Input light position significantly influences WFS outcomes in MMF.
    • A center input position yields higher contrast foci, crucial for detailed imaging.
    • An edge input position is more effective in mitigating focus aberrations.
    • Demonstrated a clear trade-off between focus contrast and aberration reduction based on input position.

    Conclusions:

    • Input light positioning is a critical parameter for optimizing MMF-based raster-scan imaging.
    • Understanding the center vs. edge input trade-off allows for tailored WFS strategies.
    • These findings are essential for advancing high-field-of-view imaging applications using MMF probes.