Related Experiment Video
Updated: Aug 9, 2026

07:42
Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases TALENs
Published on: June 20, 2016
8.4K
A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls
Konstantinos Sousounis1, Katharine Courtemanche1, Jessica L Whited2,3,4
1Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|October 22, 2022
Abstract
None:
Clustered regularly interspaced short palindromic repeats (CRISPR) is a powerful tool that enables editing of the axolotl genome. In this chapter, we will cover how to retrieve gene sequences, confirm annotation, design CRISPR targets, analyze indels, and screen for Crispant axolotls. This is a comprehensive guide on how to use CRISPR on your favorite gene and gain insights into its function.
Related Concept Videos
Homologous Recombination
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
CRISPR and crRNAs
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

