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Updated: Aug 24, 2025
06:52
Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
Published on: May 21, 2018
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Inflammasome and Inflammatory Programmed Cell Death in Chromoblastomycosis
Carla Pagliari1, Luciane Kanashiro-Galo2, Mirian Nacagami Sotto2
1Laboratório da Disciplina de Patologia de Moléstias Transmissíveis, Departamento de Patologia, Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP, Brasil. cpagliari@usp.br.
Mycopathologia
|October 23, 2022
Summary
Chromoblastomycosis (CBM) involves inflammasome elements like caspase 1, IL-1β, and IL-18 in skin lesions. These findings suggest inflammasome-mediated pyroptosis may be a therapeutic target for CBM.
Area of Science:
- Immunology
- Dermatology
- Mycology
Background:
- Chromoblastomycosis (CBM) is a chronic fungal skin infection.
- Lesions show granulomas and a Th2 cytokine pattern.
- The inflammasome pathway is crucial for inflammation and cell death.
Purpose of the Study:
- To investigate inflammasome components (caspase 1, IL-1β, IL-18, NLRP1) and RIPK3 in CBM skin lesions.
- To explore the role of inflammasome and pyroptosis in CBM pathogenesis.
Main Methods:
- Analysis of 35 CBM skin biopsies and 10 normal skin controls.
- Immunohistochemical staining for target proteins.
- Histopathological examination of lesions.
Main Results:
- Increased expression of caspase 1, IL-1β, and IL-18 in CBM inflammatory infiltrates.
- RIPK3 and NLRP1 expression were less frequent.
- Histopathology revealed characteristic epidermal and dermal changes.
Conclusions:
- The presence of caspase 1, IL-1β, IL-18, and NLRP1 suggests inflammasome activation and pyroptosis in CBM.
- Inflammasome-mediated responses could be a potential therapeutic target for CBM.
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