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Updated: Aug 24, 2025

An Integrated Approach for Microprotein Identification and Sequence Analysis
Published on: July 12, 2022
The LCD-Composer webserver: high-specificity identification and functional analysis of low-complexity domains in
Sean M Cascarina1, Eric D Ross1
1Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA.
Summary:
Low-complexity domains (LCDs) in proteins are regions enriched in a small subset of amino acids. LCDs exist in all domains of life, often have unusual biophysical behavior, and function in both normal and pathological processes. We recently developed an algorithm to identify LCDs based predominantly on amino acid composition thresholds. Here, we have integrated this algorithm with a webserver and augmented it with additional analysis options. Specifically, users can (i) search for LCDs in whole proteomes by setting minimum composition thresholds for individual or grouped amino acids, (ii) submit a known LCD sequence to search for similar LCDs, (iii) search for and plot LCDs within a single protein, (iv) statistically test for enrichment of LCDs within a user-provided protein set and (v) specifically identify proteins with multiple types of LCDs.
Availability And Implementation:
The LCD-Composer server can be accessed at http://lcd-composer.bmb.colostate.edu. The corresponding command-line scripts can be accessed at https://github.com/RossLabCSU/LCD-Composer/tree/master/WebserverScripts.
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