Related Experiment Video
Updated: Aug 23, 2025
DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
Published on: September 27, 2019
Duplex-Specific Nuclease-Enabled Target Recycling on Semiconducting Metal-Organic Framework Heterojunctions for
Ge Gao1,2, Jia-Hao Chen1, Cheng-Jun Li1
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing210023, China.
Abstract:
Semiconductor metal-organic frameworks (MOFs) and heterojunctions have gained increasing attention in many fields, yet their full potential remains largely unexplored. Advanced optobioelectronics are envisioned to create more opportunities for innovative biomedical applications. This study reports a UiO-66-NH2 (U6N)/CdS quantum dots (QDs)-gated organic photoelectrochemical transistor (OPECT) and its application toward energy-transfer-based sensitive microRNA-166a (miRNA-166a) detection assisted by duplex-specific nuclease (DSN)-enabled target recycling. Specifically, a U6N/CdS QDs photoanode was fabricated and shown to be efficiently gating a poly(3,4-ethylenedioxythiophene) doped with poly(styrene sulfonate) (PEDOT/PSS) channel, while the DSN-enabled release of Au-reporters and hybridization upon the U6N/CdS QDs photoanode could significantly inhibit the photoanode response via an energy transfer process and thus modulate the device response, permitting novel dual-amplified optobioelectronic miRNA-166a detection with a low detection limit of 1.0 fM. This work not only features the DSN-amplified miRNA detection via an OPECT route but also unveils the potential of semiconductor MOF heterojunctions for futuristic optobioelectronics.
