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A Simple Method for Inducing Masculinization of Zebrafish Stocks Using 17α-Methyltestosterone
Jennifer L Matthews1, Joy Murphy1, Andrzej Nasiadka1
1Zebrafish International Resource Center, University of Oregon, Eugene, Oregon, USA.
Zebrafish
|November 1, 2022
Abstract:
Severely skewed sex ratios in zebrafish stocks can pose significant hurdles for line propagation and sperm cryopreservation. To overcome female-biased sex ratios in stocks derived from imported sperm samples, the Zebrafish International Resource Center has implemented routine supplementation of larval food with 17α-methyltestosterone to skew gonadal sex differentiation toward masculinization. Resulting stocks averaged 80% males.
