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Efficient Pathogen Capture and Sensing Promoted by Dynamic Deformable Nanointerfaces.

Ying Cao¹, Na Wu¹, Hui-Da Li¹

¹Department of Chemistry, Research Center for Analytical Sciences, College of Sciences, Northeastern University, Box 332, Shenyang, 110819, China.

Small (Weinheim an Der Bergstrasse, Germany)

|November 3, 2022

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Researchers developed a novel nanointerface using M13 bacteriophage (M13 phage) for highly sensitive pathogen detection. This engineered interface significantly improves pathogen capture and detection limits, offering a promising tool for diagnostics.

Area of Science:

Bionanomaterials
Nanotechnology
Biosensing

Background:

M13 bacteriophage (M13 phage) is a versatile bionanomaterial with modifiable properties.
Existing detection methods often lack sensitivity and efficiency.

Purpose of the Study:

To fabricate a dynamic, deformable nanointerface using M13 phage for ultrasensitive pathogen detection.
To enhance pathogen capture affinity and improve assay sensitivity.

Main Methods:

Genetically modifying M13 phage PIII proteins to display 6His peptide for anchoring.
Utilizing M13 phage pVIII proteins to display aptamers and complementary strands (c-apt).
Employing rolling circle amplification (RCA) initiated by target pathogen binding.

Main Results:

Keywords:

M13 bacteriophages Staphylococcus aureus deformable interfaces rolling circle amplification (RCA)

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Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

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Achieved a 19-fold improvement in capture affinity due to flexible M13 nanofibers.
Demonstrated a three-orders of magnitude improvement in sensitivity.
Established a detection limit of 8 colony-forming units per milliliter (cfu mL⁻¹) for Staphylococcus aureus.

Conclusions:

The deformable M13 phage nanointerface significantly enhances pathogen capture and assay performance.
The engineered interface provides inspiration for developing more efficient reaction interfaces.
This approach offers a highly sensitive method for pathogen detection.