Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

92.2K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
92.2K
RNA-seq03:21

RNA-seq

10.3K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.3K
Sanger Sequencing01:57

Sanger Sequencing

756.0K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
756.0K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

6.5K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
6.5K
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

13.8K
13.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

2025 White Paper on Recent Issues in Bioanalysis: What is the Future of Bioanalytical LIMS? AI/ML Integration in Bioanalysis; Tear Sample Collection; Radiolabeled Mass Balance Studies; Chiral Assays; Bioanalysis of Antibody-Oligonucleotide & Bicycle Drug Conjugates (<u>PART 1A</u> - Recommendations on Mass Spectrometry Assays, Chromatography, Sample Preparation and Regulated Bioanalysis Sampling, Validating, Analyzing & Reporting <u>PART 1B</u> - Regulatory Agencies' Input on Regulated Bioanalysis/BMV).

Bioanalysis·2026
Same author

Targeted BDNF upregulation via upstream open reading frame disruption.

Molecular therapy : the journal of the American Society of Gene Therapy·2025
Same author

uORF-targeting steric block antisense oligonucleotides do not reproducibly increase RNASEH1 expression.

Molecular therapy. Nucleic acids·2025
Same author

Non-uniform dystrophin re-expression after CRISPR-mediated exon excision in the dystrophin/utrophin double-knockout mouse model of DMD.

Molecular therapy. Nucleic acids·2022
Same author

Improvements to Hybridization-Ligation ELISA Methods to Overcome Bioanalytical Challenges Posed by Novel Oligonucleotide Therapeutics.

Nucleic acid therapeutics·2022
Same author

Development of a sensitive trial-ready poly(GP) CSF biomarker assay for <i>C9orf72</i>-associated frontotemporal dementia and amyotrophic lateral sclerosis.

Journal of neurology, neurosurgery, and psychiatry·2022
Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Aug 23, 2025

3' End Sequencing Library Preparation with A-seq2
12:01

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

10.7K

Full-Length Transcript Phasing with Third-Generation Sequencing.

Nenad Svrzikapa1,2, Ramakrishna Boyanapalli3

  • 1Wave Life Sciences, Cambridge, MA, USA. nsvrzikapa@wavelifesci.com.

Methods in Molecular Biology (Clifton, N.J.)
|November 6, 2022
PubMed
Summary
This summary is machine-generated.

Accurately determining repeat expansion lengths and haplotypes is crucial for diagnosing genetic diseases. This study presents a method for haplotype phasing of the HTT gene, adaptable for other repeat expansion disorders.

Keywords:
Allele-selective targetingAntisense oligonucleotidesClinical haplotypingFull-length transcriptGenetic medicinesHaplotype phasingLong-read sequencingRepeat expansion disordersRepeat expansions

More Related Videos

Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer
09:38

Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer

Published on: March 28, 2018

12.4K
Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq
09:26

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq

Published on: July 10, 2019

10.7K

Related Experiment Videos

Last Updated: Aug 23, 2025

3' End Sequencing Library Preparation with A-seq2
12:01

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

10.7K
Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer
09:38

Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer

Published on: March 28, 2018

12.4K
Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq
09:26

Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing RIPiT-Seq

Published on: July 10, 2019

10.7K

Area of Science:

  • Genetics
  • Molecular Biology
  • Neurology

Background:

  • Repeat expansions in simple tandem repeat sequences cause over 40 neurological disorders.
  • Disease onset and severity correlate with expanded polymorphic repeats beyond a threshold.
  • Haplotype-specific targeting is key for precision genetic medicine in dominant disorders.

Purpose of the Study:

  • To address challenges in accurate length and haplotype determination of repeat expansions.
  • To present a method for targeted haplotype phasing of the HTT gene.
  • To enable application of this method to other full-length transcripts and repeat expansion disorders.

Main Methods:

  • Development of custom methods to overcome limitations of next-generation sequencing (NGS) read lengths.
  • Targeted haplotype phasing approach.
  • Focus on the HTT gene for demonstration.

Main Results:

  • A method for targeted haplotype phasing of the HTT gene was developed.
  • The method is designed to accurately determine repeat expansion lengths and haplotypes.
  • The approach is adaptable for other genetic conditions.

Conclusions:

  • Accurate haplotyping of full-length transcripts is vital for genetic disease diagnosis and treatment.
  • The presented method offers a solution for haplotype phasing in repeat expansion disorders.
  • This technique holds promise for advancing precision genetic medicine.