Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

3.8K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
3.8K
Nuclear Magnetic Resonance (NMR): Overview01:07

Nuclear Magnetic Resonance (NMR): Overview

2.9K
Nuclear magnetic resonance (NMR) is a phenomenon exhibited by certain nuclei that can absorb characteristic radio frequency radiation under certain conditions. NMR has been extensively applied in molecular spectroscopy and medical diagnostic imaging. In both these applications, the molecule or subject under study is placed in a magnetic field and irradiated with radio frequency energy.
NMR spectroscopy generates a spectrum where the characteristic absorption frequencies of the sample are...
2.9K
NMR Spectrometers: Overview01:20

NMR Spectrometers: Overview

1.3K
NMR spectrometers consist of a strong magnet, a radiofrequency transmitter, and a detector attached to a computer console for recording spectra of samples containing NMR-active nuclei. In first-generation NMR instruments called continuous-wave spectrometers, the resonance frequencies of the nuclei are determined by frequency-sweep or field-sweep methods. The magnetic field strength is fixed and the rf signal is swept in the former, while the radiofrequency signal is fixed and the magnetic field...
1.3K
Proteomics01:33

Proteomics

7.7K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.7K
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

276
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
276
Two-Dimensional (2D) NMR: Overview01:12

Two-Dimensional (2D) NMR: Overview

804
The 1D NMR spectrum of large and complex molecules like natural products has complicated splitting patterns and overlapping signals, which can be easily interpreted using 2-dimensional (2D) NMR. Unlike 1D NMR, 2D NMR has two frequency axes that provide the coupling information between the nucleus A and nucleus B in a molecule. The process from which 2D spectra are obtained has four steps.
The first step is the preparation period, during which nucleus A is excited with a radiofrequency pulse....
804

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reply to Quillien: Intuitive preferences and interpretive humility in intentionality judgments.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Solution Structure of the Broad-Spectrum Bacteriocin Garvicin Q.

International journal of molecular sciences·2025
Same author

Recommendations for sample selection, collection and preparation for NMR-based metabolomics studies of blood.

Metabolomics : Official journal of the Metabolomic Society·2025
Same author

Belief in belief: Even atheists in secular countries show intuitive preferences favoring religious belief.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Biased expectations about future choice options predict sequential economic decisions.

Communications psychology·2024
Same author

'So Help Me God'? Does oath swearing in courtroom scenarios impact trial outcomes?

British journal of psychology (London, England : 1953)·2023
Same journal

Endothelial Cell Phenotypic Plasticity in Atherosclerosis.

Handbook of experimental pharmacology·2026
Same journal

Endothelial Dysfunction and Neurovascular Alterations in Autism Spectrum Disorder.

Handbook of experimental pharmacology·2026
Same journal

Molecular Mechanisms of Endothelial Shear Stress Mechanotransduction in Health and Disease.

Handbook of experimental pharmacology·2026
Same journal

Microvasculature of the Pancreatic Islets of Langerhans in Health and Diabetes.

Handbook of experimental pharmacology·2026
Same journal

Mechanisms of Actions of Physiological, Pharmacological, and Toxicological Dietary Bioactive Inorganic Boron.

Handbook of experimental pharmacology·2026
Same journal

BNCT Plus Luminescence: New Paradigm for Boron-Containing Drug Design.

Handbook of experimental pharmacology·2026
See all related articles

Related Experiment Video

Updated: Aug 22, 2025

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

621

Metabolomics and NMR.

Ryan T McKay1

  • 1Department Chemistry, College of Natural and Applied Sciences, University of Alberta, Edmonton, AB, Canada. ryan.mckay@ualberta.ca.

Handbook of Experimental Pharmacology
|November 10, 2022
PubMed
Summary
This summary is machine-generated.

This manuscript explains Nuclear Magnetic Resonance (NMR) spectroscopy, moving beyond its "black-box" use. Understanding NMR spectroscopy offers advantages and helps avoid common experimental issues.

Keywords:
AutomationBiochemistryLiquidsMetabolitesMetabolomicsNMRNuclear magnetic resonanceQuantitationSmall moleculeSolvent suppressionSpectrometry

More Related Videos

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics
13:16

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

Published on: July 31, 2021

2.0K
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.0K

Related Experiment Videos

Last Updated: Aug 22, 2025

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics
11:02

Identification and Quantification of Deranged Metabolites in Critically Ill Patients Using NMR-Based Metabolomics

Published on: November 29, 2024

621
Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics
13:16

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics

Published on: July 31, 2021

2.0K
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
14:18

A Strategy for Sensitive, Large Scale Quantitative Metabolomics

Published on: May 27, 2014

21.0K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy

Background:

  • Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful analytical technique.
  • Often treated as a "black-box" method, limiting deeper understanding and application.
  • Potential for improved experimental design and data interpretation is frequently overlooked.

Purpose of the Study:

  • To encourage a deeper understanding of NMR spectroscopy beyond its routine application.
  • To highlight key aspects of NMR spectroscopy for broader accessibility.
  • To provide references for further exploration of NMR spectroscopy principles and applications.

Main Methods:

  • Concise overview of interesting NMR spectroscopy topics.
  • Inclusion of references for advanced study at each stage.
  • Emphasis on practical aspects, including common problem avoidance.

Main Results:

  • Demonstration of the advantages of understanding NMR spectroscopy.
  • Clarification of how to avoid common experimental pitfalls.
  • Guidance on essential spectrometer information for reproducible results.

Conclusions:

  • A deeper understanding of NMR spectroscopy enhances its utility.
  • Proactive problem-solving in NMR experiments is achievable with better knowledge.
  • Standardized reporting of spectrometer details is crucial for result verification.