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Quantification of Protein Palmitoylation by Cysteine-SILAC
Caiyun Fang1, Xiaoqin Zhang2, Haojie Lu3
1Department of Chemistry, Fudan University, Shanghai, China. fangcaiyun@fudan.edu.cn.
Methods in Molecular Biology (Clifton, N.J.)
|November 12, 2022
Abstract:
Cysteine-SILAC enables the detection and quantification of protein S-palmitoylation, an important protein posttranslational modification. Here we describe the cell culture, protein extraction, selective enrichment, mass spectrometry, and data analysis for palmitoylated proteins from cell samples by this method.
Keywords:
Cysteine-SILACMass spectrometryMetabolic labelingProtein palmitoylationSelective enrichment
